Optimization of Boiling Time for Maximum Curcuminoid Yield in a Traditional Turmeric Tamarind Jamu Using UV-Vis Spectrophotometry: Optimasi Waktu Perebusan untuk Hasil Kurkuminoid Maksimum dalam Jamu Kunyit Asam Jawa Tradisional Menggunakan Spektrofotometri UV-Vis

aditya raharjo

doi:10.30595/jhepr.v4i1.378

Authors

aditya raharjo universitas muhammadiyah purwokerto

DOI:

https://doi.org/10.30595/jhepr.v4i1.378

Keywords:

Boiled herbs, Curcuma rhizome, Curcuminoid, Spectrophotometry, Jamu

Abstract

Introduction: Turmeric tamarind herbal drink (Jamu Kunyit Asam) is a popular traditional medicine in Indonesia, valued for the health benefits of curcuminoids from turmeric. However, traditional preparation methods, particularly boiling time, are not standardized, potentially leading to inconsistent curcuminoid content, which affects efficacy.

Objective: This study aimed to determine the effect of boiling time on the curcuminoid content in Jamu Kunyit Asam and to identify the optimal boiling time to achieve the maximum concentration.

Methods: Fresh curcuma rhizomes (Curcuma longa L.) and tamarind fructus (Tamarindus indica L.) were boiled at 90 ± 2°C. Samples were collected at 5, 10, 15, 20, and 30-minute intervals. Curcuminoids were isolated using liquid-liquid extraction with chloroform and quantified using a validated UV-Vis spectrophotometry method at a maximum wavelength of 423 nm.

Results: The analytical method was validated and showed excellent linearity (r = 0.9997), accuracy (92% recovery), and precision (%RSD = 0.89). The results demonstrated a non-linear relationship between boiling time and curcuminoid content. The concentration increased sharply to a peak of 57.602 ppm at the 10-minute mark, followed by a consistent decrease to 24.576 ppm by 30 minutes.

Conclusion: The optimal boiling time for maximizing curcuminoid content in Jamu Kunyit Asam is 10 minutes at 90°C. Boiling for less than 10 minutes results in incomplete extraction, while boiling longer leads to significant thermal degradation of curcuminoids. This finding provides a scientific basis for standardizing the preparation of this traditional herbal medicine to ensure optimal bioactive compound content.

References

Mukherjee, S. & K. Kar, S. Curcuminoids: The Novel Molecules of Nature. in Herbs and Spices - New Processing Technologies (IntechOpen, 2021). doi:10.5772/intechopen.99201.

Amalraj, A., Pius, A., Gopi, S. & Gopi, S. Biological activities of curcuminoids, other biomolecules from turmeric and their derivatives - A review. J Tradit Complement Med 7, 205–233 (2017).

Kurniawan, F. Y., Jalil, M., Purwantoro, A., Daryono, B. S. & Purnomo. Jamu Kunir Asem: Ethnomedicine Overview by Javanese Herbal Medicine Formers in Yogyakarta. Jurnal Jamu Indonesia 6, 8–15 (2021).

Marwasariaty, Hasna & Asmy, U. STUDI LITERATURE: EFEKTIVITAS PEMBERIAN MINUMAN KUNYIT ASAM TERHADAP PENURUNAN DISMENOREA PADA REMAJA PUTRI. JURNAL ILMIAH KEPERAWATAN ALTRUISTIK 7, 27–33 (2024).

Diniatik, D., Kustiawan, P. M. & Suryadini, H. Standardized Herbal Extract for Wound Healing: A Comparative Study of Centella asiatica, Curcuma domestica, and Heterotrigona itama Honey Combination in Rabbits. Borneo Journal of Pharmacy 7, 404–411 (2024).

Athala, S. Efektivitas Gastroprotektif Rimpang Kunyit (Curcuma Domestica Val) Pada Lambung Yang Di Induksi Aspirin. Jurnal Ilmiah Kesehatan Sandi Husada 10, 402–407 (2021).

Yusuf, H. Y. EFEKTIVITAS PENGGUNAAN KURKUMIN TERHADAP PENINGKATAN SISTEM IMUN TUBUH. Dharmakarya 12, 40 (2023).

Mohajeri, M., Momenai, R., Karami-Mohajeri, S., Ohadi, M. & Raeisi Estabragh, M. A. Curcumin as a Natural Therapeutic Agent: A Rapid Review of Potential Clinical Uses and Mechanisms of Action. Iran J Pharm Res 24, e156983 (2025).

Suprihatin, T., Rahayu, S., Rifa’i, M. & Widyarti, S. Senyawa pada Serbuk Rimpang Kunyit (Curcuma longa L.) yang Berpotensi sebagai Antioksidan. Buletin Anatomi dan Fisiologi 5, 35–42 (2020).

Putu Haridas Chandra Gayatri, Ni Made Widi Astuti & Komang Ayu Ratih Tri Bhuwana Putri. Review Aktivitas Kandungan Senyawa Aktif Rimpang Genus Curcuma Sebagai Antiinflamasi. Prosiding Workshop dan Seminar Nasional Farmasi 3, 260–270 (2025).

Patala, R., Paula, I. & Sakina, N. Aktivitas Analgesik dan Antiinflamasi Nanoemulsi Ekstrak Etanol Rimpang Kunyit (Curcuma longa L.) secara In Vivo. Journal of Pharmaceutical and Sciences 6, 1795–1803 (2023).

Compound summary: Curcuminoid.

Fitriansyah, S. N., Riasari, H., Melati, N. & Simanjuntak, N. AKTIVITAS ANTIOKSIDAN DAN KADAR TOTAL FENOL DARI EKSTRAK JAMU BERAS KENCUR DENGAN PENAMBAHAN RIMPANG KUNYIT. Pharma Xplore : Jurnal Sains dan Ilmu Farmasi 9, 14–22 (2024).

Mahardani, O. T. & Yuanita, L. EFEK METODE PENGOLAHAN DAN PENYIMPANAN TERHADAP KADAR SENYAWA FENOLIK DAN AKTIVITAS ANTIOKSIDAN. Unesa Journal of Chemistry 10, 64–78 (2021).

Yun, C.-I., Kim, M.-A., Jung, S. K., Kim, Y.-J. & Lee, J. Thermal processing effects on phytochemical profiles, antioxidant activity, and anti-inflammatory potential of extracts from dried rhizomes of Curcuma longa L. evaluated by UHPLC–MS/MS and in vitro assays. Food Chem 496, 146756 (2025).

Jain, S. & Zhong, Q. Enhanced acid stability and bioaccessibility of curcumin-loaded nanodispersions prepared with shellac, gum arabic, and Tween 20. Int J Biol Macromol 320, 146003 (2025).

Beers, S.-Jane. Jamu : The Ancient Indonesian Art of Herbal Healing. (Tuttle Pub., 2012).

Azwanida NN. A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Med Aromat Plants (Los Angel) 04, (2015).

Diana, S. PENGARUH LAMA PEREBUSAN TERHADAP AKTIVITAS ANTIOKSIDAN HERBA MENIRAN (Phyllanthus Niruri L.) DENGAN METODE DPPH. (2021).

Firdaus, M., Nazaruddin, N. & Cicilia, S. Efek Lama Perebusan terhadap Aktivitas Antioksidan Air Rebusan Batang Brotowali (Tinospora crispa L.). Journal of Food and Agricultural Product 1, 71 (2021).

I Gusti Ngurah Arry Putra, Ni Luh Ari Yusasrini & I Wayan Rai Widarta. PENGARUH LAMA PEREBUSAN TERHADAP KARAKTERISTIK LOLOH DON PIDUH (Centella asiatica L.). Jurnal Ilmu dan Teknologi Pangan 8, 189–196 (2019).

A. K. Slavova-Kazakova, L. Koleva, V.D. Kancheva & G. Delogu. Comparative study of antioxidant potential of curcumin and its degradation products– vanillin, ferulic acid and dehydrozingerone. Bulgarian Chemical Communications 50, 158–163 (2018).

Suprapto, S. & Baroroh, V. Study of curcumin degradation using UV-VIS spectrophotometry and density functional theory. in 050014 (2024). doi:10.1063/5.0211262.

Wang, Y.-J., Pan, M.-H., Cheng, A.-L., Lin, L.-I., Ho, Y.-S., Hsieh, C.-Y. & Lin, J.-K. Stability of curcumin in buffer solutions and characterization of its degradation products. J Pharm Biomed Anal 15, 1867–1876 (1997).

Farida, I. N. & Muliya, A. Validasi Kurkumin Hasil Isolasi Rimpang Kunyit Dengan Kromatografi Cair Kinerja Tinggi Photodiode Array Detector. Jurnal Pengelolaan Laboratorium Pendidikan 5, 50–57 (2023).