Water Extraction Optimization of Rice Leaf Extracts via Face-Centered Composite Design (FCCD)
DOI:
https://doi.org/10.30595/rice.v4i1.260Keywords:
Rice leaf extract, Phenolic compounds, Decoction, Response surface methodology, Antioxidant activityAbstract
Rice leaves (Oryza sativa L.) contain a high concentration of bioactive compounds, particularly flavonoids and phenolics, which have potential applications in functional foods. This study aims to optimize the decoction process for extracting phenolic compounds from rice leaves using Response Surface Methodology (RSM). A Face-Centered Composite Design (FCCD) was applied to evaluate the impact of extraction parameters, including time and liquid-to-solid ratio, on total phenolic content (TPC) and antioxidant activities (FRAP, DPPH, ABTS). Results showed that the pH of the extracts remained stable (6–7) regardless of extraction conditions, while colorimetric analysis indicated that a lower liquid-to-solid ratio and prolonged boiling enhanced yellowness (b*) and chroma. The optimal extraction conditions determined through RSM were a boiling time of 30 minutes and a ratio of 37 mL/g, yielding maximum TPC and antioxidant activities. The model demonstrated statistical significance (p < 0.05), with high R² values (>0.9) and adequate precision (>4). Under these conditions, phenolic extraction efficiency improved by 3–7% compared to previous studies, while solvent usage was reduced by 7.5%. These findings confirm that optimizing decoction parameters enhances the efficiency of phenolic compound extraction while maintaining food-grade suitability, making it feasible for large-scale applications.References
M. Sirijan et al., “Flavone and volatile metabolite profiling of rice (Oryza sativa L.) leaf tea: a multivariate approach to identify potential bioactive compounds,” Research Square, Jul. 03, 2024. https://www.researchsquare.com/article/rs-4576630/v1 (accessed Jan. 12, 2025).
A. Chahyadi and Elfahmi, “The influence of extraction methods on rutin yield of cassava leaves (Manihot esculenta Crantz),” Saudi Pharm. J., vol. 28, no. 11, pp. 1466–1473, Nov. 2020, doi: 10.1016/j.jsps.2020.09.012.
N. E. H. Lezoul et al., “Extraction Processes with Several Solvents on Total Bioactive Compounds in Different Organs of Three Medicinal Plants,” Molecules, vol. 25, no. 20, p. 4672, Oct. 2020, doi: 10.3390/molecules25204672.
A. Y. Aydar, “Utilization of Response Surface Methodology in Optimization of Extraction of Plant Materials,” in Statistical Approaches With Emphasis on Design of Experiments Applied to Chemical Processes, no. March, V. Silva, Ed. USA: InTech, 2018, pp. 157–169.
J. Giacometti, G. Žauhar, and M. Žuvić, “Optimization of ultrasonic-assisted extraction of major phenolic compounds from olive leaves (Olea europaea L.) using response surface methodology,” Foods, vol. 7, no. 9, Sep. 2018, doi: 10.3390/foods7090149.
W. L. Zhang et al., “Hydrolysis of glycosidic flavonoids during the preparation of Danggui Buxue Tang: An outcome of moderate boiling of Chinese herbal mixture,” Evidence-based Complement. Altern. Med., vol. 2014, 2014, doi: 10.1155/2014/608721.
D. Ayuni, L. Neri, P. Pittia, S. Sirikantaramas, S. Devahastin, and C. Borompichaichartkul, “Impact of extraction methods on bioactive compounds and antioxidant activities of Thai jasmine rice leaf extracts,” Int. J. Food Sci. Technol., vol. 59, no. 10, pp. 7865–7872, Oct. 2024, doi: 10.1111/ijfs.17062.
G. D. Sabatino, D. Ayuni, P. Pittia, M. Faieta, and C. Borompichaichartkul, “Optimization of microencapsulation process for jasmine rice leaves extract via spray drying,” Dry. Technol., vol. 0, no. 0, pp. 1–11, 2024, doi: 10.1080/07373937.2024.2332935.
W. Tchabo, Y. Ma, E. Kwaw, H. Zhang, X. Li, and N. A. Afoakwah, “Effects of Ultrasound, High Pressure, and Manosonication Processes on Phenolic Profile and Antioxidant Properties of a Sulfur Dioxide-Free Mulberry (Morus nigra) Wine,” Food Bioprocess Technol., vol. 10, no. 7, pp. 1210–1223, Jul. 2017, doi: 10.1007/s11947-017-1892-5.
V. Santarelli, L. Neri, K. Carbone, V. Macchioni, and P. Pittia, “Use of conventional and innovative technologies for the production of food grade hop extracts: Focus on bioactive compounds and antioxidant activity,” Plants, vol. 11, no. 1, p. 41, 2022, doi: 10.3390/plants11010041.
A. Hamad, S. Suriyarak, S. Devahastin, and C. Borompichaichartkul, “A novel approach to develop spray-dried encapsulated curcumin powder from oil-in-water emulsions stabilized by combined surfactants and chitosan,” J. Food Sci., vol. 85, no. 11, pp. 3874–3884, Nov. 2020, doi: 10.1111/1750-3841.15488.
D. Ayuni et al., “Extraction methods comparison and optimization for isoorientin and isovitexin from Thai jasmine rice leaves,” J. Food Meas. Charact., vol. 18, no. 11, pp. 9423–9434, Nov. 2024, doi: 10.1007/s11694-024-02890-3.
Issutarti, M. Devi, A. Martiningtyas, and A. Millati, “Analysis of Chemical and Physical Properties of Boiling Time in Kecombrang Drink (Etlingera Elatior, Jack),” IOP Conf. Ser. Earth Environ. Sci., vol. 1012, no. 1, p. 012034, Apr. 2022, doi: 10.1088/1755-1315/1012/1/012034.
S. Insang, I. Kijpatanasilp, S. Jafari, and K. Assatarakul, “Ultrasound-assisted extraction of functional compound from mulberry (Morus alba L.) leaf using response surface methodology and effect of microencapsulation by spray drying on quality of optimized extract,” Ultrason. Sonochem., vol. 82, p. 105806, 2022, doi: 10.1016/j.ultsonch.2021.105806.
D. P. Xu, J. Zheng, Y. Zhou, Y. Li, S. Li, and H. Bin Li, “Ultrasound-assisted extraction of natural antioxidants from the flower of Limonium sinuatum: Optimization and comparison with conventional methods,” Food Chem., vol. 217, pp. 552–559, Feb. 2017, doi: 10.1016/j.foodchem.2016.09.013.
S. Aryal, M. K. Baniya, K. Danekhu, P. Kunwar, R. Gurung, and N. Koirala, “Total Phenolic content, Flavonoid content and antioxidant potential of wild vegetables from western Nepal,” Plants, vol. 8, no. 4, Apr. 2019, doi: 10.3390/plants8040096.
P. Kashyap, D. Shikha, M. Thakur, and A. Aneja, “Functionality of apigenin as a potent antioxidant with emphasis on bioavailability, metabolism, action mechanism and in vitro and in vivo studies: A review,” J. Food Biochem., vol. 46, no. 4, pp. 1–23, Apr. 2021, doi: 10.1111/jfbc.13950.
I. G. Munteanu and C. Apetrei, “Analytical methods used in determining antioxidant activity: A review,” Int. J. Mol. Sci., vol. 22, no. 7, p. 3380, 2021, doi: 10.3390/ijms22073380.
J. Rumpf, R. Burger, and M. Schulze, “Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins,” Int. J. Biol. Macromol., vol. 233, no. January, 2023, doi: 10.1016/j.ijbiomac.2023.123470.
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